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Research Fields:
Current research interests focus on:
  --analytical biochemistry;
  --molecular diagnosis;
  --biosensors and chips;
  --clinic immunoassay;
  --cell analytical chemistry;
  --nanobiosensing and nanobiotechnology;
  --electroanalysis;
  --luminescence analysis;
  --capillary electrophoresis;
  --applications of PCR technology.

Highlight works  >>  
    This group has published 584 papers (522 papers in SCI journals), including 255 papers in journals with IF>5, and authored 35 patents, 3 English books, 7 Chinese books, 17 chapters for 9 Chinese and 8 English books. His research works have been cited by SCI journals for 21987 times (20964 times by other authors), with an h-index of 81 (Google search: 26472 citation,h-index 89) up to Aug 11, 2017.
State Key Laboratory of Analytical Chemistry for Life Science,
School of Chemistry & Chemical Engineering, Nanjing University,
Tel: +86-25-89683593 (O) 
E-mail: hxju@nju.edu.cn
Summery of Research Works in HuangxainJu¡¯s Group since 1997£º

This group is focusing their research on bioanalysis by signal amplification for interface behavior study and signal extraction, molecular recognition and their interaction, detection and tracing of biomolecules. In 1997 this group as one of the earliest groupsintroduced nanotechnology to biosensing field, and then proposed a series of novel methods for studying the direct electrochemistry of proteins and preparing novel electrochemical biosensors, which have been extensively applied and cited. In 2000 this group combined for the first time PCR technology with electroanalytical chemistry and prepared several highly sensitive DNA biosensors. They then developed the electrochemical cytosensing field, presented the methods for exogenous effect study of cell viability and electrochemical antitumor drug sensitivity test, and proposed the first ECL biosensor based on quantum dots for the first application in bioanalysisin 2003-2005. In 2005-2008 they presented five strategies for chemiluminescent multiplex immunoassay. After 2007, they presented a new ECL mechanism based on the energy transfer of quantum dots for sensitive biosensing and proposed a creative method for fracture sampling on silicone/quartz capillary microchip, designed a method for facile synthesis of highly luminescent CdTe QDs in aqueous solution and tried to develop some protocols for in-situ monoring of cell surface carbohydrates. Since 2010, this group has designed a series ofnanotheranostics for photodynamic therapy and real-time therapeutic monitoring of tumors,and signal amplification strategies with nanotechnologies and molecular biological technologies for sensitive detection of small biomolecules, proteins (tumor markers), genes (DNA and RNA), tumor cells and the carbohydrate sites on cell surfaces and for in situ analysis and function studies of intracellular functional species such as microRNA, carbohydrates, telomerase et al. by electrochemical, optical, mass spectrometric, and imaging analysis.The current research fields in this group include bioanalytical chemistry, biosensors and chips,nanobiotechnology and molecular diagnosis. The main contributions of this group can be summarized as follows:

1) Nanobiotechnology and nanoanalysis. Since this group as one of the earliest groupsintroduced nanotechnology to biosensing field in 1997, a series of signal amplification strategies based on functional nanomaterials have been designed for biosensing (Chem Soc. Rev.2012, 41, 2122). Several novel nanocomposites, nanoparticles films, nanocage, conductive ormosil materials, functionalized ordered nanoporous silica and protein-nanoparticles architecture were prepared for preparation of novel biosensors (Anal.Chem.2002, 74, 3579; Adv. Mater.2004, 16, 1189; Adv. Mater.2006, 18, 1838;Adv. Funct. Mater.2007, 17, 585). These materials greatly improve the performance of biosensors for different biomolecules.Two facile methods for preparation of surface protein-imprinted nanowires and nanoparticles were developed for protein specific recognition (Adv. Funct. Mater.2007, 17, 3223;Anal. Chem.2009, 81,9651). Recently, this group prepared several cell-specific nanoparticle for highly selective near-infrared photodynamic therapy against cancer (J. Am. Chem. Soc. 2013, 135, 18850; Angew. Chem. Int. Ed. 2014, 53, 9544; Chem. Sci. 2015, 6, 5969) and cell-subtype specific siRNA delivery(Nat. Commuun. 2016, 7, 13580).

2) Immunosensing and protein analysis. They widened the application of immunoassay by preparing a series of novel amperometricimmunosensorsand their arrays as well as an instrument for development of new immunoassay and clinic diagnostic methods (Anal. Chem.2003, 75,5429; Clin. Chem. 2007, 53, 1495; Clin. Chem. 2008, 54, 1481; Adv. Funct. Mater. 2011, 21, 2938;Anal. Chem. 2013, 85, 6505; Anal. Chem. 2014, 86, 7494; Anal. Chem. 2016, 88, 9856). Especially, an electric field-driven strategy, two accelerating strategies for fast multiplexed detection of protein biomarkers (Anal. Chem. 2008, 80, 6072; Anal. Chem. 2009, 81, 4043), four new resolution strategies for chemiluminescent multiplex immunoassay and several relevant detection systems were presented for early screening of tumors (Anal. Chem. 2006, 78, 6999;Adv. Funct. Mater. 2008, 18, 3991;Anal. Chem. 2009, 81, 5484; Anal. Chem. 2012, 84, 2410,Anal. Chem. 2014, 86, 5573; Chem. Sci. 2015, 6, 2602). This group also developed for the first time the quantitative methods for MALDI-TOF mass spectrometric detection of peptide and protease activity (Anal. Chem. 2014, 86, 8275; Anal. Chem. 2015, 87, 4409) and designed a chip for mass spectroscopicpatterning of caspase activities (Angew. Chem. Int. Ed.2016, 55, 6667).

3) Cytosensing and in situ detection of functional biomolecules. Several novel protocols based on a surface-confined carbohydrate strategy (J. Am. Chem. Soc. 2008, 130, 7224), cytosensor array (Angew. Chem. Int. Ed.2009, 48, 6465;Anal. Chem. 2010, 82, 5804; Anal. Chem. 2012, 84, 1452) and signal amplification strategies for convenient in situ evaluation of cell surface carbohydrate sites of interest and protein-specific glycosylation information were presented (Chem. Sci. 2015, 6, 3769; Chem. Sci. 2016, 7, 569; Angew. Chem. Int. Ed. 2016, 55, 5220). The representative work was reviewed as founding work for this field by Dr Strano from MIT (Acc. Chem. Res. 2014, 47, 979). A series of methods for monitoring of cell adhesion, exogenous effect study of cell viability and electrochemical antitumor drug sensitivity test were developed. A method for highly sensitive detection ofcirculating tumor cells was developed (Clin. Cancer Res. 2007, 13, 2992). The in situ detection methods for intracellular microRNA, telomerase, sialyltransferase activity and caspase family were proposed (Angew. Chem. Int. Ed.2012, 51, 4607,J. Am. Chem. Soc. 2013, 135, 13282;J. Am. Chem. Soc. 2014, 136, 8205; Sci. Rep. 2015, 5, 10947; Chem. Sci. 2015, 6, 3365).

4) Bioanalysis based on electrochemiluminescence of quantum dots. Their works brought a new field in biosensing application of quantum dots. The first ECL sensor for H2O2 (Anal. Chem. 2004, 76, 6871) and the first ECL biosensor for glucose (Chem. Commun. 2007, 404) based on the intrinsic ECL emission of quantum dots were proposed. An electrogenerated precursor was firstly designed for facile synthesis of highly luminescent QDs in aqueous solution (Chem. Commun.2008, 450), and several methods for ECL detection of biomolecules, including the first coreactant for extremely sensitive anodic ECL of QDs, were developed (Anal. Chem. 80, 5377-5382 (2008);Chem. Commun.2010, 4, 404; Chem. Commun. 2013, 49, 2106; Anal. Chem. 2016, 88, 845).Newmechnasims, inclusing intermediate annihilation (Anal. Chem. 2007, 79, 6690), the firstECL energy transfer mechanism(Anal. Chem. 2007, 21, 8055), surface unpassivation(Anal. Chem.2010, 82, 3359), self-produced coreactant (Anal. Chem. 2010, 82, 7351), coreactant consumption (Anal. Chem. 2011, 83, 5214; Anal. Chem. 2013, 85, 5390), electron and energy transfer (Chem. Commun. 2013, 49, 2106),target-induced structure switching (Anal. Chem. 2014,86, 8735) and ratiometricelectrochemiluminescence (Anal. Chem. 2014, 86, 5158) for ECL biosensing and signal amplification have also been proposed.

5) Molecular biological amplification. Some electrochemical hybridization biosensors with detection limits down to 1.7¡Á10-20mol DNA were designed for detection of short-stranded HBV DNA by combining a PCR procedure at the beginning of this century. By introducing rolling circle amplification(Anal. Chem. 2010, 82, 3337; Anal. Chem.2012, 84, 7166), target-induced repeated primer extension (Chem. Commun.2012, 48, 10216), hybridization chain reaction (Anal. Chem. 2013, 85, 11788), target DNA recycling amplification such as endonuclease- (Chem. Eur. J. 2012, 18, 13871; Chem. Commun. 2013, 49, 7561), exonuclease- (Chem. Commun.2013, 49, 7561) and polymerase-based circular strand-replacement polymerization (Anal. Chem. 2012, 84, 4587;Biosens. Bioelectron.2013, 39, 199), and assistant DNA recycling (Chem. Commun. 2013, 49, 4006) to amplify the electrochemical, optical and visual signals, this group has established a series methods for convenient and ultrasensitive detections of small biomolecules, proteins, DNA (Chem. Sci. 2015, 6, 4318), RNA and microRNA. This groupsummarized the functions and detection methods of microRNA (Chem. Rev. 2013, 113, 6207¨C6233).
 

Nature Publishing Group commented the 2013 Nature Index of Prof. Ju's Group in Nature Index China on Dec. 18, 2014(doi:10.1038/ 516S66a) as following: NJU is by far Nanjing¡¯s largest contributor to the Nature Index, and fourth overall in China by WFC. In 2013, the WFC is 194.6. NJU derives most of its WFC from chemistry. Huangxian Ju, Jingjuan Xu and Hongyuan Chen are NJU¡¯s largest contributors. Ju produced 13 articles (WFC=11.8). Xu and Chen co-authered 9 articles (WFC = 8.4).

       According to the ranking of top authors in the documents,Biomed Experts(www.biomedexperts.com)listed the study of Ju¡¯s group as the top in the field of biosensing and antibody fixation, ranking the second in the immunoassay, the third in the field of electrochemistry and the seventh in quantum dots. The screen shots is shown as follows:


 
 

 

 

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School of chemistry & Chemical Engineering, Nanjing University
Dr. Leech Key Lab of Analytical Chemistry for Life Science, Ministry of Education


 

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maintained by Dr H.X. Ju on Aug. 11, 2017